地下水中多菌灵残留的SPE-HPLC分析方法
Determination of carbendazim residues in groundwater by SPE-HPLC
云南民族大学学报:自然科学版,2015,24(5):365-368

杨永军 YYJ

摘要


建立了检测地下水中多菌灵残留量的固相萃取-高效液相色谱法.样品经C18固相萃取小柱富集净化后,以甲醇-(水+氨水)为流动相,高效液相色谱-紫外检测器检测,外标法定量.在0.01~1.0mg/L质量浓度范围内,线性相关系数0.9999,最低检出限为0.0034mg/L.添加质量浓度为0.01,0.05,0.18mg/L,回收率在95.3%~97.8%之间,相对标准偏差(RSD)为3.09%~4.18%.方法应用在实际样品分析中可行,适用于地下水样中痕量有机物的检测. The determination method of carbendazim residues in groundwater by SPE-HPLC was studied.The samples were processed by C18 Solid-Phase Extraction(SPE)column,with mobile phase of methanol-(water and ammonia),high-performance liquid chromatography-UV detector and the residues were quantified external standard calibration curve method.The linearity was kept in the range from 0.01—1.0mg/L,with correlation coefficient 0.9999.The limits of detection was 0.0034mg/L.The recovery studies were performed at the three spike levels of 0.01,0.05 and 0.18mg/L,the mean recoveries were between 95.3% and 97.8% with a relative standard deviation(RSD)ranging from 3.09% to 4.18%.The method was practicably analysing the actual sample,and it was suitable for detecting trace of organisms in groundwater.

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