RP-HPLC法测定复方岩白菜素片中岩白菜素的含量
Determination of the content of bergeninum in compound bergeninum tablets by RP-HPLC
云南民族大学学报:自然科学版,2015,24(3):199-201

何耀莹 HRY

摘要


建立了复方岩白菜素片中岩白菜素含量测定的反相高效液相色谱法.采用色谱柱Diamonsil C18(5μm,46mm×250mm),柱温为30℃;流动相为V(乙腈)∶V(水)=15∶85;流速为1.0mL·min^-1;检测波长272nm.研究结果表明:岩白菜素在28.32~226.56μg/ml的范围内呈良好的线性关系,样品平均回收率为100.86%,RSD=0.89%.该方法操作简便,快速,具有良好的稳定性和重复性,适用于复方岩白菜素片中岩白菜素含量测定. This research aims to establish a RP-HPLC method for the determination of the content of bergeninum in compound bergeninum tablets. The column used is Diamonsil C18(5μm,4.6mm×250mm) and its temperature is 30℃. The mobile phase is acetonitrile-water(15:85) with a flow rate of 1.0mL·min^-1. The detection wavelength is 272nm. The experiment result shows that bergeninum has a good linear correlation in the range of 28.32μg/mL—226.56μg/mL. The average recovery is 100.86% and the RSD is 0.89%. The method is simple and quick with good stability and repeatability, and suitable for the determination of the content of bergeninum in compound bergeninum tablets.

参考



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