SDS-PAGE电泳法测定蒜头果蛋白的相对分子质量
Determination of the relative molecular mass of malanin with SDS-PAGE
云南民族大学学报:自然科学版,2015,24(2):123-125

袁燕 YY

摘要


蒜头果蛋白(malanin)是从广西、云南特有植物蒜头果中分离、纯化出的一种新的、具有抗肿瘤活性的植物蛋白质.采用变性十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)测定malanin的相对分子质量.结果表明,malanin是一种双链蛋白质,由A、B 2条肽链通过二硫键连接而成,其相对分子质量约为65.94×10^3.方法具有简单、快速、直观的特点. Malanin,a novel plant protein with anti-tumor activities,was isolated and purified from an endemic plant of malania oleifera in Guangxi and Yunnan provinces. The relative molecular mass of malanin was determined by the denaturing sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE). The result has showed that malanin is a protein with two chains,chain-A and chain-B,which are cross-linked by one or two disulfide bonds,and its relative molecular mass is about 65.94×10^3. SDS-PAGE gel electrophoresis is a simple,fast,direct-viewing method to determine the relative molecular mass of malanin.

参考



全文: PDF      下载: 1216      浏览: 357


counter for myspace
云南民族大学学报(自然科学版) 1991—2016 Copyright
地址:云南省昆明市一二.一大街134号 邮编:650031 全国邮发代号:64-47
电话:0871-65132114 传真:0871-65137493 Email:ynmzxyxb@163.com